LOCUS       pCB301_plasmid_p        4622 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Plant transformation vector pCB301 plasmid pXT1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Tao X, Yao M, Hu Z, Wei Q, Feng Z.
  TITLE     Construction of Agrobacterium-mediated Cucumber Mosaic Virus 
            Infectious cDNA Clones and 2b Deletion Viral Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Tao X, Yao M, Hu Z, Wei Q, Feng Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) Department of Plant Pathology, Nanjing 
            Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu 
            210095, P.R.China
REFERENCE   3  (bases 1 to 4622)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4622)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4622)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-MAY-2011) Department of Plant Pathology, Nanjing Agricultural
            University, No.1 Weigang Street, Nanjing, Jiangsu 210095, P.R.China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        95..767
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    768..800
                     /label=multiple cloning site region
                     /note="multiple cloning site region"
     ncRNA           800..887
                     /product="HDV-RZ"
                     /label=ribozyme
                     /note="from hepatitis delta virus"
                     /ncRNA_class="ribozyme"
     terminator      898..1150
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1198..1222
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1377..2522)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2824..3615)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(3824..4455)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4535..4559
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcagtc aacatggtgg agcacgacac tctcgtctac tccaagaata
       61 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      121 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      181 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaaa
      241 gaatgcctct accgacagtg gtcccaaaga tggacccccc acccacgagg aacatcgtgg
      301 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg
      361 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg
      421 ctattgagac tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      481 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      541 ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac cgacagtggt cccaaagatg
      601 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      661 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      721 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggcct gacctgcagg
      781 tcgactctag aggatccccg ggtcggcatg gcatctccac ctcctcgcgg tccgacctgg
      841 gcatccgaag gaggacgtcg tccactcgga tggctaaggg agagctcgaa tttccccgat
      901 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
      961 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     1021 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     1081 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     1141 ttactagatc ggaattcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
     1201 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     1261 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacagga
     1321 gatctcagta aagcgctggc tgaaccccca gccggaactg accccacaag gccctagcgt
     1381 ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac
     1441 tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg
     1501 cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag
     1561 tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg
     1621 tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac
     1681 gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc
     1741 ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg
     1801 gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac
     1861 gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac
     1921 accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg
     1981 ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg
     2041 gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc
     2101 gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac
     2161 gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc
     2221 ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc
     2281 tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc
     2341 agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct gggccatcga gccgacggac
     2401 tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg
     2461 gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc
     2521 attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga
     2581 tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc
     2641 ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat
     2701 accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg
     2761 atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta
     2821 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     2881 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     2941 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     3001 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     3061 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     3121 aaatggggtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     3181 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     3241 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     3301 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     3361 ccagccatca tgccgttcaa ggtgcaggac ctttggaaca ggcagctttc cttccagcca
     3421 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     3481 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     3541 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     3601 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     3661 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaactttaaa
     3721 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     3781 gagccgattt tgaaaccaca attatgggtg atgctgcaac tcgagcgggc cgggagggtt
     3841 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
     3901 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
     3961 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
     4021 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
     4081 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
     4141 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
     4201 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
     4261 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
     4321 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
     4381 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
     4441 agcccggtga gcgtctctag tggactgatg ggctgcctgt atcgagtggt gattttgtgc
     4501 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     4561 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggtt
     4621 tt
//
